Concurrent Session 8: Cell and Tissue Imaging

Concurrent Session

Date/Time: Friday, April 30, 2021 - 2:45 PM to 4:45 PM
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Description:

Synergies Between Microstructure Imaging Modalities

Light- and electron-microscopy have provided insights into the relationships between the structure and organisation of the extracellular matrix, of its normal physiological functions and of the changes occurring during growth and degeneration. However as the biological questions become more sophisticated such as investigating cell-matrix interactions and exploring mechanics on a nano-scale the limitations of these techniques become more apparent. A number of remedies are available and will be reviewed in this presentation.   In the visible-light regime multiphoton techniques such as second harmonic generation and two photon fluorescence offer sub-micron spatial resolution and sufficient depth penetration to allow the examination of unfixed mm-sized tissue specimens. Spectral analysis of light scattered inelstically from the tissue provides micron-scale maps of chemical composition (Raman scattering) or elastic moduli (Brillouin scattering). In the X ray regime the ability of small and wide angle scattering to provide structural information on the fibrous proteins from the molecular up to the microscopic scale is now well established and synchrotron sources provide timely advances in imaging speed and spatial resolution, sufficient to allow studies on relatively large specimens and to observe the changes arising from modifications in the physical environment. Other advances such as 3D phase contrast X ray microtomography are also closing the technological gap between visible- and x ray regimes. There is therefore rich promise of new biological insights, perhaps particularly if some of the modalities can be delivered using optical fibre technology to facilitate applications in intact joints.  

Followed by abstract presentations.

Abstract(s):

  • 61. AUTOMATED MORPHOLOGICAL PHENOTYPING TO CHARACTERIZE KNEE OSTEOARTHRITIS PROGRESSION
    N. K. Namiri, J. Lee, B. Astuto, F. Liu, R. Shah, S. Majumdar, V. Pedoia; Dept. of Radiology and BioMed. Imaging and Ctr. for Intelligent Imaging, Univ. of California, San Francisco, San Francisco, CA

  • 62. ASSOCIATIONS OF BONE MARROW LESIONS RELATED SUBCHONDRAL OSTEOCLASTOGENESIS AND NEUROGENESIS WITH CLINICAL SYMPTOMS IN KNEE OSTEOARTHRITIS
    F. Zhou, Z. Yu; Shanghai Ninth People's Hosp.,Shanghai Jiao Tong Univ. Sch. of Med., Shanghai, China

  • 63. SUBCHONDRAL BONE NORMALIZATION AFTER KNEE JOINT DISTRACTION TREATMENT AS MEASURED WITH CT
    M. P. Jansen1, A. Ooms1, T. D. Turmezei2,3, J. W. MacKay3,4, S. C. Mastbergen1, F. P. Lafeber1; 1Univ. Med. Ctr. Utrecht, Utrecht, Netherlands, 2Norfolk & Norwich Univ. Hosp., Norwich, United Kingdom, 3Univ. of East Anglia, Norwich, United Kingdom, 4Univ. of Cambridge, Cambridge, United Kingdom

  • 64. Imaging CD64 AS MARKER FOR MOLECULAR IMAGING OF SYNOVITIS IN OSTEOARTHRITIS
    I. Di Ceglie1, N. van Kooten1, D. Bos2, P. Jimenez-Royo3, M. van den Bosch1, A. Blom1, M. Koenders1, P. Laverman2, P. Van Lent1; 1Experimental Rheumatology, Radboud Univ. Med. Ctr., Nijmegen, Netherlands, 2Dept. of Radiology and Med. Imaging, Radboud Univ. Med. Ctr., Nijmegen, Netherlands, 3Clinical Pharmacology and Experimental Med., GlaxoSmithKline, Stevenage, United Kingdom

  • 65. MRI-BASED SUBCHONDRAL TRABECULAR BIOMARKERS AND TIBIOFEMORAL BONE 3D MORPHOLOGY CHANGES: LONGITUDINAL ANALYSIS FROM OSTEOARTHRITIS INITIATIVE
    F. Pishgar1, A. Ashraf-ganjouei2, A. Guermazi3, F. W. Roemer3, M. Jarraya4, S. Demehri1; 1Johns Hopkins Univ. Sch. of Med., Baltimore, MD, 2Tehran Univ. of Med. Sci., Tehran, Iran, Islamic Republic of, 3Boston Univ. Sch. of Med., Boston, MA, 4Harvard Med. Sch., Boston, MA

  • 66. TRABECULAR BONE TEXTURE ANALYSIS OF CONVENTIONAL RADIOGRAPHS FOR THE PREDICTION OF TOTAL KNEE REPLACEMENT RISK: DATA FROM THE OSTEOARTHRITIS INITIATIVE COHORT
    A. Almhdie-Imjabbar1,2, H. Toumi1,2, E. Lespessailles1,2; 1Univ. of Orleans, Orleans, France, 2Hosp. of Orleans, Orleans, France

Speaker(s):