Concurrent Session 8: Cell and Tissue Imaging
Date/Time: Friday, April 30, 2021 - 2:45 PM to 4:45 PM
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Synergies Between Microstructure Imaging Modalities
Light- and electron-microscopy have provided insights into the relationships between the structure and organisation of the extracellular matrix, of its normal physiological functions and of the changes occurring during growth and degeneration. However as the biological questions become more sophisticated such as investigating cell-matrix interactions and exploring mechanics on a nano-scale the limitations of these techniques become more apparent. A number of remedies are available and will be reviewed in this presentation. In the visible-light regime multiphoton techniques such as second harmonic generation and two photon fluorescence offer sub-micron spatial resolution and sufficient depth penetration to allow the examination of unfixed mm-sized tissue specimens. Spectral analysis of light scattered inelstically from the tissue provides micron-scale maps of chemical composition (Raman scattering) or elastic moduli (Brillouin scattering). In the X ray regime the ability of small and wide angle scattering to provide structural information on the fibrous proteins from the molecular up to the microscopic scale is now well established and synchrotron sources provide timely advances in imaging speed and spatial resolution, sufficient to allow studies on relatively large specimens and to observe the changes arising from modifications in the physical environment. Other advances such as 3D phase contrast X ray microtomography are also closing the technological gap between visible- and x ray regimes. There is therefore rich promise of new biological insights, perhaps particularly if some of the modalities can be delivered using optical fibre technology to facilitate applications in intact joints.
Followed by abstract presentations.