Concurrent Session 4: Cell and Gene Therapy

Concurrent Session

Date/Time: Thursday, April 29, 2021 - 2:45 PM to 4:45 PM
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Description:

Cartilage Regeneration by Activated Skeletal Stem Cells
Osteoarthritis (OA) is a degenerative disease resulting in irreversible, progressive destruction of articular cartilage. The etiology of OA is complex and involves a variety of factors, including genetic predisposition, acute injury and chronic inflammation. Here we investigate the ability of resident skeletal stem-cell (SSC) populations to regenerate cartilage in relation to age, a possible contributor to the development of osteoarthritis. We demonstrate that aging is associated with progressive loss of SSCs and diminished chondrogenesis in the joints of both mice and humans. However, a local expansion of SSCs could still be triggered in the chondral surface of adult limb joints in mice by stimulating a regenerative response using microfracture (MF) surgery. Although MF-activated SSCs tended to form fibrous tissues, localized co-delivery of BMP2 and soluble VEGFR1 (sVEGFR1), a VEGF receptor antagonist, in a hydrogel skewed differentiation of MF-activated SSCs toward articular cartilage. These data indicate that following MF, a resident stem-cell population can be induced to generate cartilage for treatment of localized chondral disease in OA.

Abstract(s):

  • 31. EXOSOMES DERIVED FROM MSCS REPAIR MENISCUS DEFECTS AND ENHANCED ENDOGENOUS CELL PROLIFERATION AND MIGRATION VIA CXCL5 AND CXCL6/CXCR2 SIGNALING
    K. Kawata1, H. Katagiri1,2, K. Tsuji3, K. Miyatake1, Y. Nakagawa1, I. Sekiya4, H. Koga1; 1Dept. of Joint Surgery and Sports Med., Tokyo Med. and Dental Univ., Tokyo, Japan, 2Dept. of orthopedics, Dokyo Med. Univ. Saitama Med. Ctr., Saitama, Japan, 3Dept. of Cartilage Regeneration, Tokyo Med. and Dental Univ., Tokyo, Japan, 4Ctr. for stem Cell and Regenerative Med., Tokyo Med. and Dental Univ., Tokyo, Japan

  • 32.┬áPROGRANULIN DERIVATIVE ATSTTRIN PROMOTES CHONDROGENESIS AND CARTILAGE REGENERATION
    K. Wang1, J. Wei2,1, C. Liu1,3; 1Dept. of Orthopaedic Surgery, New York Univ. Med. Ctr., New York, NY, 2Qilu Hosp. of Shandong Univ., Jinan, China, 3Dept. of Cell Biology, New York Univ. Sch. of Med., New York, NY

  • 33. DIFFERENTIAL MICRORNA PROFILE OF MESENCHYMAL STROMAL CELLS IDENTIFIES TARGET GENES WHICH IMPROVE THERAPEUTIC EFFICACY IN THE CONTEXT OF OSTEOARTHRITIS
    R. Rabani1,2, J. Chahal1,2, N. Mahomed1,2, R. Gandhi1,2, S. Viswanathan1,2; 1Krembil Res. Inst., Univ. Hlth.Network, Toronto, Toronto, ON, Canada, 2Osteoarthritis Res. Program, Div. of Orthopedic Surgery, Schroeder Arthritis Inst., Univ. Hlth.Network, Toronto, ON, Canada

  • 34. MULTI-OMIC PROFILING REVEALS DISTINCT MICRORNA, LONG NON-CODING RNA, CIRCULAR RNA, AND GENE INTERACTOMES IN LATE RADIOGRAPHIC STAGE KNEE OSTEOARTHRITIS PATIENTS
    A. Ratneswaran1,2, C. Pastrello1,2, A. S. Ali1,2, P. Potla1,2, O. Espin-Garcia1,2, S. Lively1,2, A. Perruccio1,2, Y. Rampersaud1,2, R. Gandhi1,2, M. Kapoor1,2; 1Schroeder Arthritis Inst., Univ. Hlth.Network, Toronto, ON, Canada, 2Krembil Res. Inst., Univ. Hlth.Network, Toronto, ON, Canada

  • 35. AGE-RELATED INCREASE IN MATRIX STIFFNESS DOWNREGULATES ╬▒-KLOTHO IN CARTILAGE AND INDUCES CARTILAGE DEGENERATION
    H. Iijima1,2, G. Gilmer3,4, K. Wang1, A. Bean1, F. Ambrosio1,5; 1Dept. of Physical Med. and Rehabilitation, Univ. of Pittsburgh, Pittsburgh, PA, 2Japan Society for the Promotion of Sci., Tokyo, Japan, 3Med. Scientist Training Program, Sch. of Med., Univ. of Pittsburgh, Pittsburgh, PA, 4Dept. of Bioengineering, Univ. of Pittsburgh, Pittsburgh, PA, 5McGowan Inst. for Regenerative Med., Univ. of Pittsburgh, Pittsburgh, PA

  • 36. HUMAN MESENCHYMAL STEM CELLS RELEASE FUNCTIONAL MITOCHONDRIA IN EXTRACELLULAR VESICLES
    M. A. Thomas, J. L. Miller, M. L. Delco; Cornell Univ., Ithaca, NY

Speaker(s):